7R27
Crystal structure of the L. plantarum D-alanine ligase DltA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.522, 53.573, 102.072 |
| Unit cell angles | 90.00, 96.78, 90.00 |
Refinement procedure
| Resolution | 47.360 - 2.010 |
| R-factor | 0.2115 |
| Rwork | 0.209 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dhv |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.360 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.171 | 1.452 |
| Number of reflections | 49012 | 3478 |
| <I/σ(I)> | 7.2 | 0.9 |
| Completeness [%] | 99.4 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350, KCl |
