7R24

Crystal structure of rat Arc CTD in complex with two anti-Arc nanobodies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	40.880, 92.810, 114.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.510 - 2.700
R-factor	0.2585
Rwork	0.257
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x3h 4x3x 6h16 4fhb
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.770
High resolution limit [Å]	2.700	2.700
Rmeas	0.293	2.552
Number of reflections	22999	885
<I/σ(I)>	6.7	1
Completeness [%]	99.2	98.2
Redundancy	7.5	7.8
CC(1/2)	0.991	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% PEG10000, 10% PEG8000