7R20
Anti-Arc nanobody E5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-21 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 45.900, 45.900, 103.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.980 - 1.420 |
| R-factor | 0.1897 |
| Rwork | 0.185 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hvg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.274 |
| Data reduction software | XDS (Feb 5, 2021) |
| Data scaling software | XSCALE (Feb 5, 2021) |
| Phasing software | PHASER (2.8.3.) |
| Refinement software | PHENIX (dev_3958) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.980 | 1.460 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmeas | 0.108 | 5.687 |
| Number of reflections | 201623 | 21801 |
| <I/σ(I)> | 9.5 | 0.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 9.2 | 9.2 |
| CC(1/2) | 0.999 | 0.333 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.26 M (NH4)2SO4, 0.1 M LiSO4 and 0.1 M Tris-HCl pH 7.5 |
