7R1O

p62-ZZ domain of the human sequestosome in complex with dusquetide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-07
Detector	MAR CCD 130 mm
Wavelength(s)	1.0403
Spacegroup name	C 1 2 1
Unit cell lengths	124.070, 25.150, 77.530
Unit cell angles	90.00, 124.92, 90.00

Refinement procedure

Resolution	24.628 - 2.202
Rwork	0.178
R-free	0.24300
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.858
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	SHELXDE (2006/3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.085	0.366
Number of reflections	10270	1487
<I/σ(I)>	10.9	3.7
Completeness [%]	98.5	99.2
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	14.4% PEG 8000, 0.08 M Na-cacodylate pH 6.5, 0.16 M Ca acetate. 200 nl 18 mg/ml p62-ZZ-dusquetide complex + 100 nl seed solution + 300 nl reservoir solution.