7R1G

Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-38 (R207C, D210S, S211V)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	Agilent SuperNova
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-28
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54056
Spacegroup name	P 21 21 21
Unit cell lengths	49.815, 74.691, 147.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.131 - 1.950
Rwork	0.248
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zal
RMSD bond length	0.009
RMSD bond angle	1.617
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.131	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.099	0.361
Rmeas	0.121	0.464
Number of reflections	38942	2596
<I/σ(I)>	7	2.1
Completeness [%]	95.3
Redundancy	2.7
CC(1/2)	0.991	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	30% PEG 4000, 0.2 M MgCl2 in 0.1M Tris-HCl pH 8.5