7R0Q
KRasG12C in complex with GDP and compound 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99995 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.230, 39.800, 62.810 |
| Unit cell angles | 76.89, 81.20, 77.32 |
Refinement procedure
| Resolution | 17.960 - 1.950 |
| R-factor | 0.24 |
| Rwork | 0.237 |
| R-free | 0.29250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7r0m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.810 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.066 | 0.353 |
| Number of reflections | 22980 | 412 |
| <I/σ(I)> | 7.66 | 2.2 |
| Completeness [%] | 92.8 | 90.1 |
| Redundancy | 1.74 | 1.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG 4000, 0.1 M TRIS HCl pH 8.5, 0.2 M CaCl2 |
