7R0M
KRasG12C in complex with GDP and JDQ443
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-29 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0000096 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.652, 39.496, 62.362 |
| Unit cell angles | 77.24, 80.85, 77.15 |
Refinement procedure
| Resolution | 60.410 - 1.611 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JXKO |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.410 | 1.800 |
| High resolution limit [Å] | 1.611 | 1.611 |
| Rmerge | 0.076 | 0.555 |
| Number of reflections | 23415 | 469 |
| <I/σ(I)> | 6.1 | 1.3 |
| Completeness [%] | 83.6 | 44.5 |
| Redundancy | 2.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 4000, 0.1 M HEPES pH 7.5, 0.2 M CaCl2 |
