7R0H
STRUCTURAL BASIS OF ION UPTAKE IN COPPER-TRANSPORTING P1B-TYPE ATPASES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.37 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 130.122, 151.115, 219.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.930 - 3.310 |
| R-factor | 0.2179 |
| Rwork | 0.214 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rfu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.391 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.429 |
| High resolution limit [Å] | 0.000 | 3.310 |
| Rmerge | 0.161 | 1.792 |
| Rmeas | 0.161 | 1.817 |
| Rpim | 0.026 | 0.300 |
| Number of reflections | 16266 | 1599 |
| <I/σ(I)> | 21.49 | 1.96 |
| Completeness [%] | 99.6 | |
| Redundancy | 38 | |
| CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 1.5 M ammonium citrate, 0.1 M MES pH=5.5 |
