7R0G
STRUCTURAL BASIS OF ION UPTAKE IN COPPER-TRANSPORTING P1B-TYPE ATPASES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.194, 226.056, 111.106 |
| Unit cell angles | 90.00, 134.06, 90.00 |
Refinement procedure
| Resolution | 48.750 - 4.010 |
| R-factor | 0.2673 |
| Rwork | 0.263 |
| R-free | 0.31170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rfu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.063 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.154 |
| High resolution limit [Å] | 4.000 | 4.011 |
| Rmerge | 0.206 | 3.855 |
| Rmeas | 0.219 | 4.099 |
| Rpim | 0.075 | 1.386 |
| Number of reflections | 36001 | 1863 |
| <I/σ(I)> | 6.88 | 0.44 |
| Completeness [%] | 93.9 | |
| Redundancy | 8.4 | |
| CC(1/2) | 0.996 | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 1.5 M ammonium citrate, 0.1 M MES pH=5.5 |
