7R08
Abortive infection DNA polymerase Abi-P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.033200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.334, 88.359, 161.094 |
| Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
| Resolution | 47.910 - 3.100 |
| R-factor | 0.2213 |
| Rwork | 0.220 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CryoEM model |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.910 | 3.280 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmeas | 0.106 | 1.240 |
| Number of reflections | 67114 | 10560 |
| <I/σ(I)> | 12.22 | |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 4.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | Well condition: 6% (v/v) 2-propanol, 26% PEG MME 550, 0.1 M sodium acetate trihydrate (pH 4.5) Protein concentration: 6 mg/mL |
