7QZO
Crystal structure of GacS D1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.152, 73.532, 104.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.790 - 1.450 |
| R-factor | 0.1321 |
| Rwork | 0.131 |
| R-free | 0.15350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mmn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.563 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.790 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 47424 | 2300 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8 | |
| CC(1/2) | 0.990 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M to 1.2 M Na acetate and 0.1 M HEPES from pH 7 to pH 8 in presence of 50 mM cadmium sulfate |
