7QZM

ATAD2 in complex with FragLite28

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.911
Spacegroup name	P 65 2 2
Unit cell lengths	79.068, 79.068, 137.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.540 - 1.450
Rwork	0.208
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dai
RMSD bond length	0.015
RMSD bond angle	2.092
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.540	68.540	1.470
High resolution limit [Å]	1.450	7.940	1.450
Rmerge	0.058	0.029	5.476
Rmeas	0.059	0.030	5.652
Rpim	0.013	0.007	1.390
Number of reflections	45657	370	2212
<I/σ(I)>	31.7
Completeness [%]	100.0
Redundancy	38	30.1	31.3
CC(1/2)	0.999	0.999	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M BisTris pH 6-7, 1.7-2.1M Ammonium sulphate