7QYS
Crystal structure of RimK from Pseudomonas syringae DC3000
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 95.905, 96.091, 156.992 |
| Unit cell angles | 88.94, 84.37, 89.97 |
Refinement procedure
| Resolution | 49.800 - 2.900 |
| R-factor | 0.2377 |
| Rwork | 0.237 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iwx |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.296 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.800 | 49.800 | 2.950 |
| High resolution limit [Å] | 2.900 | 15.880 | 2.900 |
| Rmerge | 0.161 | 0.048 | 0.976 |
| Rmeas | 0.192 | 0.059 | 1.174 |
| Rpim | 0.102 | 0.034 | 0.644 |
| Total number of observations | 2249 | 19555 | |
| Number of reflections | 122285 | 726 | 6015 |
| <I/σ(I)> | 5.8 | 13.4 | 1.1 |
| Completeness [%] | 98.9 | 96.9 | 98.6 |
| Redundancy | 3.4 | 3.1 | 3.3 |
| CC(1/2) | 0.983 | 0.989 | 0.430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | NULL |
