7QY5
Crystal structure of the S.pombe Ars2-Red1 complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-07 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.670, 128.281, 89.261 |
| Unit cell angles | 90.00, 108.08, 90.00 |
Refinement procedure
| Resolution | 84.850 - 2.770 |
| R-factor | 0.2314 |
| Rwork | 0.228 |
| R-free | 0.29770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | alpafold |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.375 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.850 | 2.994 |
| High resolution limit [Å] | 2.770 | 2.771 |
| Rmerge | 0.290 | |
| Number of reflections | 26786 | 1339 |
| <I/σ(I)> | 5.3 | 1.5 |
| Completeness [%] | 76.0 | 18.4 |
| Redundancy | 5.56 | 6.2 |
| CC(1/2) | 0.980 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 278.15 | 200mM Ammonium Formate, 20% PEG3350 |
