7QX4
mosquitocidal Cry11Aa determined at pH 7 from naturally-occurring nanocrystals by Serial femtosecond crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2018-02-26 |
| Detector | CS-PAD CXI-2 |
| Wavelength(s) | 1.27 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.640, 155.690, 171.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.550 - 2.600 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.24120 |
| Structure solution method | SAD |
| Data reduction software | CrystFEL (0.8.0) |
| Data scaling software | CrystFEL (0.8.0) |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.19.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.550 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 24198 | 1583 |
| <I/σ(I)> | 9.5 | 1.16 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 341.09 | 230.58 |
| CC(1/2) | 1.000 | 0.380 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 7 | 300 | In cell crystallization by recombinant expression in the Bacillus thuringiensis serovar israelensis strain 4Q7 devoid of its pBt plasmid. |
