7QWK
GCN2 (EIF2ALPHA KINASE 4, E2AK4) IN COMPLEX WITH COMPOUND 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.799, 77.795, 101.690 |
| Unit cell angles | 89.86, 90.05, 68.58 |
Refinement procedure
| Resolution | 42.000 - 2.300 |
| Rwork | 0.214 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyd |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.670 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.151 | 0.773 |
| Number of reflections | 90485 | 3929 |
| <I/σ(I)> | 3.6 | 0.9 |
| Completeness [%] | 96.3 | 85 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM Bis Tris, 20 to 30 percent PEG3350, 200mM ammonium sulfate |
