7QW8
Adenine-specific DNA methyltransferase M.BseCI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.700, 85.700, 151.800 |
| Unit cell angles | 90.00, 95.10, 90.00 |
Refinement procedure
| Resolution | 24.710 - 2.500 |
| R-factor | 0.1714 |
| Rwork | 0.170 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7qw5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.697 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.710 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.047 | 1.000 |
| Number of reflections | 46250 | 4485 |
| <I/σ(I)> | 11.1 | 3.4 |
| Completeness [%] | 97.3 | 94.2 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.6 | 290 | PEG, MES, ammonium sulfate |
