7QW7
Adenine-specific DNA methyltransferase M.BseCI complexed with AdoHcy and cognate fully methylated DNA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2014-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97951 |
Spacegroup name | P 61 |
Unit cell lengths | 88.010, 88.010, 155.481 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.010 - 2.600 |
R-factor | 0.1991 |
Rwork | 0.198 |
R-free | 0.21670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7qw5 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.894 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.010 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.113 | 2.317 |
Rmeas | 0.130 | 2.683 |
Rpim | 0.063 | 1.325 |
Number of reflections | 19732 | 2101 |
<I/σ(I)> | 5.1 | 0.7 |
Completeness [%] | 94.1 | 82.4 |
Redundancy | 4.2 | 4.1 |
CC(1/2) | 0.927 | 0.189 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | Glycerol ethoxylate, Tris-HCl |