7QVR
Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-37 (G206S, R207T, D210S)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-05 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.224, 74.112, 147.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.281 - 1.900 |
| Rwork | 0.223 |
| R-free | 0.28830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zal |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.657 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.281 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.327 |
| Rmeas | 0.097 | 0.487 |
| Number of reflections | 42970 | 2773 |
| <I/σ(I)> | 9 | 2.1 |
| Completeness [%] | 95.6 | 89.8 |
| Redundancy | 2.6 | |
| CC(1/2) | 0.992 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 6000, 0,2 M MgCl2 in 0.1M Tris-HCl pH 8.5 |
