7QV0

Covalent complex between Scalindua brodae amxFabZ and amxACP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99988
Spacegroup name	P 32 2 1
Unit cell lengths	94.789, 94.789, 158.218
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.400 - 2.490
R-factor	0.2424
Rwork	0.241
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6Y52
RMSD bond length	0.005
RMSD bond angle	0.881
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.400	2.800
High resolution limit [Å]	2.490	2.490
Rmerge	0.077	0.942
Number of reflections	17797	927
<I/σ(I)>	13.9	1.8
Completeness [%]	92.1
Redundancy	7
CC(1/2)	0.999	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	10% (w/v) PEG 3350, 0.2 M ammonium acetate, 0.1 M sodium acetate