7QU8

ADGRG3/GPR97 Extracellular Region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	96.278, 96.278, 172.838
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.109 - 3.370
Rwork	0.266
R-free	0.31300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kvm
RMSD bond length	0.005
RMSD bond angle	1.229
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.280	3.640
High resolution limit [Å]	3.370	3.370
Rmerge	0.027	0.123
Rmeas	0.028	0.129
Rpim	0.083	0.036
Number of reflections	12030	2398
<I/σ(I)>	6.5
Completeness [%]	99.7	99.4
Redundancy	11.6	12.2
CC(1/2)	0.996	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	1.8 M Ammonium citrate