7QU2

Junin virus GP1 glycoprotein in complex with Fab fragment of antibody JUN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	69.847, 66.752, 73.101
Unit cell angles	90.00, 103.50, 90.00

Refinement procedure

Resolution	67.920 - 2.500
R-factor	0.2247
Rwork	0.223
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nuz
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.920	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.131	0.794
Number of reflections	22859	1100
<I/σ(I)>	7.1
Completeness [%]	99.8	96.2
Redundancy	6.5	5.9
CC(1/2)	0.995	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% w/v PEG8000 and 0.1 M HEPES (pH 7.5)