7QTW

Kaposi sarcoma associated herpes virus(KSHV) encoded apoptosis inhibitor, KsBcl-2 in complex with Bid BH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 64
Unit cell lengths	89.476, 89.476, 51.056
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.740 - 1.410
R-factor	0.1824
Rwork	0.181
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alpha fold generated model for this protein sequence
RMSD bond length	0.013
RMSD bond angle	1.132
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.740	1.460
High resolution limit [Å]	1.410	1.410
Number of reflections	44937	308
<I/σ(I)>	8.7
Completeness [%]	100.0
Redundancy	19.3
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1 M Sodium acetate pH 4.6 8% PEG 4000