7QTU

Structural biology of the NS1 avian influenza protein subversion on the Scribble cell polarity module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	C 1 2 1
Unit cell lengths	77.836, 77.716, 64.752
Unit cell angles	90.00, 94.16, 90.00

Refinement procedure

Resolution	42.940 - 2.840
Rwork	0.252
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vwi
RMSD bond length	0.004
RMSD bond angle	0.757
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER (1.15.2-3472-000)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.940	2.990
High resolution limit [Å]	2.840	2.840
Rmerge	0.069	0.370
Number of reflections	8916	864
<I/σ(I)>	9.6
Completeness [%]	96.9
Redundancy	3
CC(1/2)	0.990	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Sodium Cacodylate pH4.2, 40% MPD