7QTN
Duplex RNA containing Xanthosine-Cytosine base pairs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 44.880, 44.880, 149.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.870 - 1.200 |
| R-factor | 0.1838 |
| Rwork | 0.182 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 433d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 1894942 | 68877 |
| <I/σ(I)> | 32.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.200 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Magnesium sulfate, TRIS HCl, Ammonium sulfate |
