7QTE
Crystal Structure of the Fe(II)/alpha-ketoglutarate dependent dioxygenase PlaO1 in complex with cobalt and succinate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-28 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000040 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 116.077, 142.910, 109.072 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.240 - 1.810 |
R-factor | 0.1538 |
Rwork | 0.153 |
R-free | 0.17580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gqw |
Data reduction software | autoPROC (1.0.5) |
Data scaling software | Aimless (0.5.25) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20-44590) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 109.070 | 109.070 | 1.910 |
High resolution limit [Å] | 1.810 | 5.730 | 1.810 |
Rmerge | 0.146 | 0.045 | 1.265 |
Rmeas | 0.151 | 0.047 | 1.319 |
Rpim | 0.041 | 0.013 | 0.371 |
Total number of observations | 1117345 | 38040 | 149153 |
Number of reflections | 82190 | 2782 | 11869 |
<I/σ(I)> | 14.9 | 43.3 | 2.3 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 13.6 | 13.7 | 12.6 |
CC(1/2) | 0.999 | 0.999 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Morpheus B7 |