7QT0
Antibody FenAb136 - fentanyl complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-19 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.982, 105.843, 172.775 |
| Unit cell angles | 90.00, 112.24, 90.00 |
Refinement procedure
| Resolution | 54.520 - 2.070 |
| R-factor | 0.2422 |
| Rwork | 0.242 |
| R-free | 0.28190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h2b |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.520 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.064 | 0.598 |
| Number of reflections | 181970 | 17881 |
| <I/σ(I)> | 7.29 | 1.2 |
| Completeness [%] | 97.5 | 96.49 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.987 | 0.584 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 281 | 2.0m M (NH4)2SO4, 0.1 M ADA/NaOH, 10 % Ethanol |
