7QSP

Permutated C-terminal lobe of the ribose binding protein from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	41.690, 41.970, 132.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.760 - 1.360
R-factor	0.1746
Rwork	0.173
R-free	0.21120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fn9
RMSD bond length	0.008
RMSD bond angle	1.040
Data reduction software	XDS (20210323)
Data scaling software	XDS (20210323)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.760	1.400
High resolution limit [Å]	1.360	1.360
Rmerge	0.081	1.907
Rmeas	0.085	2.008
Rpim	0.026	0.616
Number of reflections	50882	4875
<I/σ(I)>	13.93	1
Completeness [%]	99.0	96.7
Redundancy	10.5	9.9
CC(1/2)	0.999	0.322

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Magnesium acetate, 20% (w/v) PEG3350, cryogenic additives: 20% PEG3000, 20% Ethylene-glycol, 0.2M KNO3