7QSP
Permutated C-terminal lobe of the ribose binding protein from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.690, 41.970, 132.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.760 - 1.360 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.21120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fn9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS (20210323) |
| Data scaling software | XDS (20210323) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.760 | 1.400 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.081 | 1.907 |
| Rmeas | 0.085 | 2.008 |
| Rpim | 0.026 | 0.616 |
| Number of reflections | 50882 | 4875 |
| <I/σ(I)> | 13.93 | 1 |
| Completeness [%] | 99.0 | 96.7 |
| Redundancy | 10.5 | 9.9 |
| CC(1/2) | 0.999 | 0.322 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Magnesium acetate, 20% (w/v) PEG3350, cryogenic additives: 20% PEG3000, 20% Ethylene-glycol, 0.2M KNO3 |
