7QSJ

Methylmannose polysaccharide hydrolase MmpH from M. hassiacum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9809
Spacegroup name	P 1 21 1
Unit cell lengths	47.037, 51.807, 149.134
Unit cell angles	90.00, 95.10, 90.00

Refinement procedure

Resolution	49.510 - 1.350
R-factor	0.144
Rwork	0.142
R-free	0.17680
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.726
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	1.398
High resolution limit [Å]	1.350	1.350
Rmerge	0.024	0.680
Rmeas	0.034	0.962
Rpim	0.024	0.680
Number of reflections	156986	15681
<I/σ(I)>	16.42	1.17
Completeness [%]	99.9
Redundancy	2
CC(1/2)	1.000	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-HCl pH 8.5, 0.02 M MgCl2, 21% (w/v) polyacrylic acid