7QSF

Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-12 (G206C, R207T, D210A, S211A)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	Agilent SuperNova
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-16
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54056
Spacegroup name	P 21 21 21
Unit cell lengths	51.623, 75.052, 149.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.050 - 1.600
Rwork	0.174
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zal
RMSD bond length	0.010
RMSD bond angle	1.672
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.050	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.056	0.220
Rmeas	0.070	0.293
Number of reflections	69731	3274
<I/σ(I)>	9.8
Completeness [%]	90.7	86.8
Redundancy	2.3	1.8
CC(1/2)	0.995	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	30% PEG 6000, 0.2 M MgCl2 in 0.1M Tris-HCl pH 8.5