7QS5

Crystal structure of B30.2 PRYSPRY domain of TRIM67

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.99981
Spacegroup name	I 41
Unit cell lengths	135.392, 135.392, 35.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.870 - 1.650
R-factor	0.1559
Rwork	0.155
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7b2s
RMSD bond length	0.016
RMSD bond angle	1.505
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.870	47.870	1.690
High resolution limit [Å]	1.650	7.380	1.650
Rmerge	0.063	0.056	0.947
Rmeas	0.069	0.059	1.050
Rpim	0.021	0.019	0.319
Number of reflections	39503	489	2917
<I/σ(I)>	17.7		2.4
Completeness [%]	99.9	99.6	100
Redundancy	10.6	10.1	10.8
CC(1/2)	0.999	0.994	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293.15	20% high molecular weight PEG Smears, 0.1M acetate pH 4.5