7QS2

Crystal structure of B30.2 PRYSPRY domain of TRIM15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00004
Spacegroup name	P 41 21 2
Unit cell lengths	54.764, 54.764, 125.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.230 - 1.700
R-factor	0.1703
Rwork	0.169
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6umb
RMSD bond length	0.015
RMSD bond angle	1.571
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.230	41.230	1.740
High resolution limit [Å]	1.700	8.500	1.700
Rmerge	0.112	0.045	1.221
Rmeas	0.123	0.048	1.174
Rpim	0.035	0.015	0.352
Number of reflections	21814	228	1267
<I/σ(I)>	15.7		2.2
Completeness [%]	100.0	99.4	100
Redundancy	12.2	9.5	12.5
CC(1/2)	0.999	0.998	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	20% PEG3350, 10% ethylene glycol, 0.1M bis-tris-propane pH 6.5, 0.2M sodium iodide