7QS1

Crystal structure of B30.2 PRYSPRY domain of TRIM11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	65.636, 42.018, 68.584
Unit cell angles	90.00, 99.44, 90.00

Refinement procedure

Resolution	43.360 - 1.930
R-factor	0.1702
Rwork	0.168
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iwg
RMSD bond length	0.015
RMSD bond angle	1.459
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.360	43.360	2.000
High resolution limit [Å]	1.930	7.470	1.930
Rmerge	0.065	0.038	0.764
Rmeas	0.078	0.042	0.921
Rpim	0.032	0.017	0.369
Number of reflections	27861	533	2730
<I/σ(I)>	12.3		2
Completeness [%]	99.2	99.4	99.4
Redundancy	5.9	5.6	6.1
CC(1/2)	0.998	0.998	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	20% PEG3350, 10% ethylene glycol, 0.2M sodium sulfate