7QS0

Crystal structure of B30.2 PRYSPRY domain of TRIM10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-04
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00002
Spacegroup name	P 32
Unit cell lengths	65.991, 65.991, 103.312
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.320 - 2.300
R-factor	0.2065
Rwork	0.204
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6umb
RMSD bond length	0.011
RMSD bond angle	1.295
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.320	38.320	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.090	0.062	0.351
Rmeas	0.101	0.066	0.401
Rpim	0.034	0.022	0.133
Number of reflections	22199	385	2174
<I/σ(I)>	3.4		1.8
Completeness [%]	99.2	97.6	100
Redundancy	8.7	9.2	9
CC(1/2)	0.997	0.997	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	25% ethylene glycol