7QRZ

Crystal structure of B30.2 PRYSPRY domain of MID2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	63.921, 75.084, 78.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.670 - 1.570
R-factor	0.1639
Rwork	0.162
R-free	0.19210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jbm
RMSD bond length	0.016
RMSD bond angle	1.654
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.670	48.670	1.610
High resolution limit [Å]	1.570	7.020	1.570
Rmerge	0.060	0.028	0.889
Rmeas	0.066	0.029	0.981
Rpim	0.020	0.009	0.287
Number of reflections	26626	345	1941
<I/σ(I)>	20.8		2.8
Completeness [%]	99.9	99.6	99.8
Redundancy	11.2	10.1	11.5
CC(1/2)	1.000	1.000	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	18% High molecular weight PEG smears, 0.1M ADA pH 6.5, 0.2M ammonium sulfate