7QRW

Crystal structure of NHL domain of TRIM3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00000
Spacegroup name	P 41 21 2
Unit cell lengths	58.933, 58.933, 161.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.580 - 1.700
R-factor	0.1839
Rwork	0.182
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7b2r
RMSD bond length	0.016
RMSD bond angle	1.538
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.580	47.580	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.058	0.039	0.587
Rmeas	0.062	0.041	0.622
Rpim	0.016	0.011	0.148
Number of reflections	31138	441	2171
<I/σ(I)>	24.6		3.8
Completeness [%]	97.1	98.8	94.5
Redundancy	16	13.6	17.3
CC(1/2)	0.999	0.999	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	2.4M AmmSO4