7QRR

Crystal structure of Noumeavirus NMV_189 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	221.567, 111.852, 127.897
Unit cell angles	90.00, 122.62, 90.00

Refinement procedure

Resolution	38.790 - 1.900
R-factor	0.1632
Rwork	0.162
R-free	0.19070
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.7.1)
Phasing software	SHELX
Refinement software	PHENIX (1.8.4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.660	46.660	1.930
High resolution limit [Å]	1.900	10.410	1.900
Rmerge	0.076	0.050	0.398
Rmeas	0.082	0.054	0.426
Rpim	0.029	0.019	0.150
Total number of observations	1558992	9670	79060
Number of reflections	202894	1290	9923
<I/σ(I)>	16.7	31.6	4.8
Completeness [%]	98.4	97.9	97.2
Redundancy	7.7	7.5	8
CC(1/2)	0.998	0.998	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	30% PEG 4000 (w/v), 10% MPD (v/v), 0.1M AmSO4, 0.1M NaCl, 0.1M NaCaco