7QRO

Crystal structure of the unconventional kinetochore protein Trypanosoma brucei KKT4 BRCT domain K543A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	46.663, 61.343, 67.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.470 - 1.800
R-factor	0.1978
Rwork	0.196
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zpk
RMSD bond length	0.009
RMSD bond angle	1.083
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.470	1.890
High resolution limit [Å]	1.800	1.800
Rmeas	0.048
Number of reflections	18591	18590
<I/σ(I)>	1.43
Completeness [%]	100.0
Redundancy	9.7
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	HEPES, sodium chloride, TCEP, sodium thiocyanate, 20% w/v PEG3350