7QRG

Structure of the post-fusion complex between precursor membrane ectodomain (prM) and envelope ectodomain protein (E) from tick-borne encephalitis virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 3 2 1
Unit cell lengths	97.935, 97.935, 115.164
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.970 - 2.800
R-factor	0.23
Rwork	0.228
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1urz 3c5x
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.238	2.730
Rmeas	0.286	3.250
Rpim	0.117	1.310
Number of reflections	15904	2299
<I/σ(I)>	6.3
Completeness [%]	98.9
Redundancy	5.7
CC(1/2)	0.990	0.306

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	293	0.1M Na Malonate, 0.1M HEPES pH 6.7, 18.9% (w/v) PEG 3350