7QRF

Structure of the dimeric complex between precursor membrane ectodomain (prM) and envelope protein ectodomain (E) from tick-borne encephalitis virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	H 3 2
Unit cell lengths	155.730, 155.730, 161.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.420 - 2.280
R-factor	0.183
Rwork	0.181
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1svb 3c5x
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS (Feb 5 2021)
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.435
High resolution limit [Å]	2.280	2.280
Rmerge	0.096	2.623
Rmeas	0.099	2.699
Rpim	0.023	0.891
Number of reflections	28985	1449
<I/σ(I)>	19.8
Completeness [%]	95.0
Redundancy	19.5
CC(1/2)	0.998	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	3.5	293	0.2M Li(SO4), 0.1M Na citrate pH 3.5, 28% (v/v) PEG 400