7QRE

Structure of the hetero-tetramer complex between precursor membrane protein fragment (pr) and envelope protein (E) from tick-borne encephalitis virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.07197
Spacegroup name	P 41 3 2
Unit cell lengths	164.320, 164.320, 164.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.540 - 2.700
R-factor	0.191
Rwork	0.188
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1svb 3c5x
RMSD bond length	0.010
RMSD bond angle	1.260
Data reduction software	XDS (Feb 5 2021)
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.560	49.560	2.830
High resolution limit [Å]	2.700	12.170	2.700
Rmerge	0.244	0.059	4.825
Rmeas	0.248	0.060	4.908
Rpim	0.043	0.013	0.890
Number of reflections	21401	303	1517
<I/σ(I)>	13.8
Completeness [%]	100.0	98.7	100
Redundancy	32.9	23.4	30
CC(1/2)	0.998	0.998	0.332

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	293	0.2M (NH4)SO4, 0.1M Na acetate pH 4.6, 28% (v/v) PEG MME 550, 2.8% (v/v) tert-Butanol