7QRD

Crystal structure of mouse CARM1 in complex with histone H3_10-25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	75.183, 99.204, 208.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.650 - 2.000
R-factor	0.224
Rwork	0.222
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20rc2_4400)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.125	3.520
Rmeas	0.136	3.520
Rpim	0.053	1.604
Number of reflections	105764	729
<I/σ(I)>	8.6
Completeness [%]	99.6	95.2
Redundancy	6.3	5.5
CC(1/2)	0.999	0.273

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20 mM Tris-HCl pH 7.5 50 mM NaCl 1 mM TCEP