7QRD
Crystal structure of mouse CARM1 in complex with histone H3_10-25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.183, 99.204, 208.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.650 - 2.000 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ih3 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20rc2_4400) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.125 | 3.520 |
| Rmeas | 0.136 | 3.520 |
| Rpim | 0.053 | 1.604 |
| Number of reflections | 105764 | 729 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.6 | 95.2 |
| Redundancy | 6.3 | 5.5 |
| CC(1/2) | 0.999 | 0.273 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20 mM Tris-HCl pH 7.5 50 mM NaCl 1 mM TCEP |
