7QR7
SpCas9 bound to AAVS1 off-target2 DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.753, 67.129, 186.633 |
| Unit cell angles | 90.00, 111.00, 90.00 |
Refinement procedure
| Resolution | 47.660 - 3.000 |
| R-factor | 0.2176 |
| Rwork | 0.216 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fq5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.564 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.660 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.220 | 2.350 |
| Number of reflections | 41411 | 4115 |
| <I/σ(I)> | 9 | 0.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.997 | 0.332 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350 |
