7QR3

Chimpanzee CPEB3 HDV-like ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	110
Detector technology	PIXEL
Collection date	2019-10-22
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00004
Spacegroup name	C 2 2 21
Unit cell lengths	120.254, 135.916, 83.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.650 - 2.180
R-factor	0.2426
Rwork	0.241
R-free	0.27690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1drz
RMSD bond length	0.002
RMSD bond angle	0.633
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.958	2.355
High resolution limit [Å]	2.180	2.180
Number of reflections	28178	1410
<I/σ(I)>	24.9
Completeness [%]	93.7
Redundancy	13.3
CC(1/2)	1.000	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	277	19-24% PEG 3350, 0.2 M sodium formate 30-200 mM HEPES-KOH pH 7.5