7QQY
yeast Gid10 bound to Art2 Pro/N-degron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-04-30 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.524, 67.817, 75.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.740 - 1.260 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ns3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.520 | 1.330 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Number of reflections | 57128 | 8755 |
| <I/σ(I)> | 26.1 | |
| Completeness [%] | 99.2 | 95.6 |
| Redundancy | 12.6 | |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 18.5% PEG3350, 0.1 M Bis-Tris propane pH 6.0 and 0.2 M potassium chloride |
