7QQX

SpCas9 bound to FANCF off-target5 DNA substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	178.534, 67.378, 188.387
Unit cell angles	90.00, 110.90, 90.00

Refinement procedure

Resolution	48.000 - 2.400
R-factor	0.1839
Rwork	0.182
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fq5
RMSD bond length	0.004
RMSD bond angle	0.624
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.940	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.120	1.720
Number of reflections	81607	8058
<I/σ(I)>	10.5	1
Completeness [%]	99.1	98.8
Redundancy	6.3	6.2
CC(1/2)	0.998	0.438

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350