7QQN
The PDZ domain of SNTG1 complexed with the acetylated PDZ-binding motif of TRPV3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.830, 132.440, 63.660 |
| Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
| Resolution | 45.467 - 2.450 |
| R-factor | 0.2221 |
| Rwork | 0.220 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n7d |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.467 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmeas | 0.222 | 2.042 |
| Number of reflections | 35752 | 2621 |
| <I/σ(I)> | 8.22 | 1.18 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.66 | 6.66 |
| CC(1/2) | 0.996 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.15 M Lithium sulfate 0.05 M Magnesium chloride hexahydrate 0.1 M HEPES 7.8 20 % v/v PEG Smear High |
