7QPR
Structure of full length SpoT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 128.791, 133.761, 211.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.880 - 2.513 |
| R-factor | 0.2227 |
| Rwork | 0.221 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6s2v |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.880 | 2.780 |
| High resolution limit [Å] | 2.513 | 2.513 |
| Rmerge | 0.180 | 1.664 |
| Rpim | 0.051 | 0.459 |
| Number of reflections | 83804 | 4191 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 95.5 | 72.6 |
| Redundancy | 13.8 | 14 |
| CC(1/2) | 0.995 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.85M sodium citrate, 0.1M sodium chloride, 0.1M tris |
