7QPO
Crystal structure of human trans-3-Hydroxy-L-proline dehydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.054, 122.923, 73.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.100 - 3.000 |
| R-factor | 0.22309 |
| Rwork | 0.221 |
| R-free | 0.26182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r77 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.397 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.100 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.082 | 0.466 |
| Number of reflections | 20658 | 2049 |
| <I/σ(I)> | 5.57 | 1.56 |
| Completeness [%] | 98.9 | 98.89 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293.15 | 0.1 M MES pH=6.5 12% (w/v) PEG 20000 |
