7QPH
Crystal structure of mouse CARM1 in complex with histone H3_22-31 K27 acetylated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.343, 99.184, 208.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.250 - 1.900 |
| R-factor | 0.1845 |
| Rwork | 0.183 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ih3 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20rc2_4400) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.250 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 1.572 |
| Rmeas | 0.085 | 1.744 |
| Rpim | 0.033 | 0.742 |
| Number of reflections | 123546 | 6017 |
| <I/σ(I)> | 11 | 1.1 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 6.5 | 5.3 |
| CC(1/2) | 0.998 | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16% PEG 3350, 0.2M ammonium sulfate, 0.1M Tris HCl pH8.5 |
